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Basic information
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| Chemical Name | HK-2 |
| Remark | HK-2细胞属源于正常肾的近曲小管细胞，通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2；Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317，最后将PA317细胞产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性，但未用G418筛选转导克隆。Southern和FISH分析显示，HK-2细胞来源于单克隆。PCR检测证实，HK-2细胞基因组中含有E6/E7基因。 |
| Storage Instructions | 气相：空气，95%；CO2，5%, 温度：37℃ |
| Cell Name | HK-2 |
Biological information
| Growth Medium | MEM（含NEAA）＋10% FBS＋1% P/S |
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| Species Reactivity | |
| Growth | 贴壁细胞 |
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| Generation Ratio | 1:2-1:4 |
| Characters | 上皮细胞样 |
Security information
| Culture Conditions | |
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| BSL | BSL-2 |
| Notes | |
| Liquid Frequency | 2-3次/周 |
Product Details
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Related Literature
丙谷二肽(L-alanyl-L-glutamine,Ala-Gln)为白色结晶性粉末,无臭无味,分子式为C8H15N3O4,相对分子质量为217.22,结构如图1所示。Ala-Gln的溶解度可达586 g/L(20℃),比游离L-Gln高20倍,略溶于冰乙酸,几乎不溶于甲醇。游离L-Gln在酸性或高温条件下极易分解并生成有毒的焦谷氨酸和氨,而Ala-Gln具有良好的热稳定性,可在120℃下不分解,Ala-Gln可在体内快速分解生成游离L-Gln,提高体内游离L-Gln含量,无副作用产生。目前认为Ala-Gln是L-Gln的最佳替代品,可克服L-Gln不易储存、水溶性差、高温易产生有毒物质等缺点,作为一种新颖的营养补充剂,已应用于临床治疗。
Publish Date:July 8, 2024
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